DATASET

AS-LS_ITS_metadata.csv (9.86 kB)

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P1-1b-ITS_S38_L001_R1_001.fastq.gz (18.18 MB)

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P1-1b-ITS_S38_L001_R2_001.fastq.gz (20.81 MB)

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P1-2b-ITS_S39_L001_R1_001.fastq.gz (24.28 MB)

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P1-2b-ITS_S39_L001_R2_001.fastq.gz (29.72 MB)

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P1-3b-ITS_S40_L001_R1_001.fastq.gz (27.56 MB)

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P1-3b-ITS_S40_L001_R2_001.fastq.gz (31.89 MB)

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P7-2b-ITS_S41_L001_R1_001.fastq.gz (17.57 MB)

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P7-2b-ITS_S41_L001_R2_001.fastq.gz (19.87 MB)

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P7-3b-ITS_S42_L001_R1_001.fastq.gz (17.99 MB)

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P7-3b-ITS_S42_L001_R2_001.fastq.gz (21.2 MB)

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P12-5-1b-ITS_S67_L001_R1_001.fastq.gz (20.33 MB)

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P12-5-1b-ITS_S67_L001_R2_001.fastq.gz (22.73 MB)

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P12-5-2b-ITS_S68_L001_R1_001.fastq.gz (17.18 MB)

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P12-5-2b-ITS_S68_L001_R2_001.fastq.gz (19.28 MB)

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P12-5-3b-ITS_S69_L001_R1_001.fastq.gz (15.14 MB)

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P12-5-3b-ITS_S69_L001_R2_001.fastq.gz (16.97 MB)

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P12-8-1b-ITS_S64_L001_R1_001.fastq.gz (19.17 MB)

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P12-8-1b-ITS_S64_L001_R2_001.fastq.gz (22.02 MB)

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P12-8-2b-ITS_S65_L001_R1_001.fastq.gz (25.93 MB)

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Assembly of plant-associated soil microbial communities during succession in dry and moist Neotropical forests - ITS (Fungi) Data

dataset

posted on 2023-04-02, 20:44 authored by Kristin SaltonstallKristin Saltonstall, Jefferson HallJefferson Hall, Michiel van BreugelMichiel van Breugel, Hilda Castillo, Wayra Navia

Our study was conducted at two sites that represent dry (Los Santos) and moist (Agua Salud) tropical lowland forest types in Panama. The two sites have distinct tree species composition and richness, with relatively little species overlap. We collected 36 surface soil samples (0 to 15 cm depth) in three land use categories (cattle pasture, 20 year old secondary forest, and old secondary forest) at each site during the wet season. Cores were sampled at 10 m intervals along four replicate transects in each of the three land use categories at each site. This soil metabarcoding dataset was created using ITS primers (ITS1F and ITS2 (Gardes & Bruns 1993, White et al. 1990)). Data were processed using the R packages DADA2, phyloseq, vegan, and microbiome. Sample metadata includes soil chemistry and alpha diversity calculations for each sample