ABIF etdir @ 2021-12-10 23:59:00.0 2021-11-09 14:49:00.0 2103668 BDTv3.1_PA_Protocol-POP7BDTv3.1_PA_Protocol-POP7 KB 1.4.1 525.0KB_3500_POP7_BDTv3.mobKB.bcpfalse00falsetruefalsetrue20false10000.0800true0.030,15300,100300, 1008,43truetrue4truetruetrue205503true20false20202020falsetrue43020false SequencingPOP75024// StdSeq50_POP7xl module edited 09/04/08
// Standard Sequencing module
//
//REGULARDC_RunModule_Comment;StdSeq50_POP7xl module edited 09/04/08;List.;STRING;nk;nk;Run Module Comment;falseOven_Temperature;60;Deg. C;INTEGER;18;65;Oven Temperature throughout the run;trueTemperature_Tolerance;3;Deg. C;INTEGER;0;50;Tolerance of the temperature that the system will use during its wait for temperature state;falseOven_Wait_Time;1800;sec.;INTEGER;1;1800;Timeout for oven wait for temperature;falseArray_Heater_Temperature;50;Deg. C;FLOAT;30.0;50.0;Detection cell heater temperature setting;falsePoly_Fill_Vol;4000;steps;INTEGER;3000;4600;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueCap_Fill_Vol;2960;steps;INTEGER;1;4600;Number of steps to pump during capillary fill;falseDC_Cap24_EPS_ARC_SELF;6;uAmps;INTEGER;1;1000;Arc current self test for 24 cap array;falseMax_Current;425;uAmps;INTEGER;1;500;Power supply current limit;falseCurrent_Stability;20.1;uAmps;FLOAT;0;1000;Max Current Variance for EP;trueVoltage_Tolerance;0.6;kVolts;FLOAT;0.0;5.0;Power supply voltage tolerance;falsePreRun_Voltage;15;kVolts;FLOAT;0;18;Pre-Run Voltage;trueDC_PreRun_Time;180;sec.;INTEGER;1;1000;Length of Pre-Run;trueInjection_Voltage;1.6;kVolts;FLOAT;1;15;Injection Voltage;trueDC_Injection_Time;15;sec.;INTEGER;1;600;Injection Time;trueInjection_Depth_Offset;0;steps;INTEGER;-500;500;Injection Depth Offset;falseVoltage_Number_Of_Steps;40;nk;INTEGER;1;100;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueVoltage_Step_Interval;15;sec;INTEGER;1;60;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueVoltage_Ramp_Wait;600;sec;INTEGER;1;1000;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;falseDC_Data_Delay;480;sec.;INTEGER;1;3600;Time delay before beginning data collection;trueRun_Voltage;8.5;kVolts;FLOAT;0;19.5;Separation (run) Voltage.;trueDC_Run_Time;5760;sec.;INTEGER;300;14000;Separation (run) Time;trueFirst_ReadOut_Time;180;msec.;INTEGER;100;5000;Sampling rate in milliseconds coming from the camera;falseSecond_ReadOut_Time;180;msec.;INTEGER;100;5000;Sampling rate in milliseconds coming from the camera;falseExposure_Time;0;msec.;INTEGER;0;5000;CCD exposure time = integration time in milliseconds;falseDC_RS_Plate_Type;$DC_RS_Plate_Type;List.;STRING;nk;nk;Plate type indicator;falseDC_RS_CSData;$DC_RS_CSData;List;STRING;nk;nk;Capillary mapping;falseDC_RS_Binning_Pattern;;pixels;STRING;;;spectral binning pattern;falseDC_Scale_Divisor;2.0;;FLOAT;;;scale divisor;falseDC_Down_Sample;2;;INTEGER;;;number of frames to average;falseDC_Laser_Power_Setting;20.0;mWatts;FLOAT;;;laser power setting;false//
//
SYSTem:MESSage "$DC_RunModule_Comment"
//
//
//
// Turning Laser On
//
SYSTem:MESSage "Turning Laser On."
LAS:POW:SETT $DC_Laser_Power_Setting
LAS:POW:TOL 1.0
LAS On
//
//
// Turning Zohana On
//
SYSTem:MESSage "Turning Detection Heater On."
DETH:TEMP:TOL 3.0
DETH:TEMP:SETT $Array_Heater_Temperature
DETH On
//
//
// Turning Oven On
//
SYSTem:MESSage "Setting Oven Temperature."
OVEN:TEMP:TOL $Temperature_Tolerance
OVEN:TEMP:SETT $Oven_Temperature
OVEN On
//
//
// Initializing Autosampler
//
SYSTem:MESSage "Initializing Autosampler."
STR Initialize
//
// Filling Polymer Pump
//
SYSTem:MESSage "Loading Waste Tray."
STR:ROB:MOV:LOAD Waste
FLU:POLY:PRES Up 0.34 50
FLU:POLY:PRES Down 0.21 200
SYSTem:MESSage "Filling Polymer Pump"
FLU:POLY:FILL $Poly_Fill_Vol
//
// Waiting for Oven to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Oven to Stabilize at Run Temperature"
OVEN:SYNC $Oven_Wait_Time
//
// Waiting for Detection Heater to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Detection Heater to Stabilize at Run Temperature"
DETH:SYNC 1800
//
// Waiting for Laser to Stabilize at Power Setting
//
SYSTem:MESSage "Waiting for Laser to Stabilize at Power Setting"
LAS:SYNC 600
//
//
// Filling Array
//
SYSTem:MESSage "Filling Array with Polymer."
FLU:CAP:FILL:THRE 150
FLU:CAP:FILL $Cap_Fill_Vol
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
//
// Bubble Check
//
SYSTem:MESSage "Testing for Normal Current."
EPS:CURR:LIM $Max_Current
EPS:CURR:ARC:THRE $Current_Stability
EPS:VOLT:SETT 1.0
EPS On
SYST:WAIT 2
EPS:CURR:ARC:SELF $DC_Cap24_EPS_ARC_SELF
EPS Off
//
// Pre Run
//
SYSTem:MESSage "PreRun"
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SETT $PreRun_Voltage
EPS On
EPS:SYNC 10
SYST:WAIT $DC_PreRun_Time
EPS Off
SYST:WAIT 3
//
//
// Rinse Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Sample injection
//
SYSTem:MESSage "Sample Injection."
STR:ROB:ZOFF $Injection_Depth_Offset
STR:CSO $DC_RS_CSData
STR:ROB:MOV:LOAD $DC_RS_Plate_Type
SYST:WAIT 15
EPS:VOLT:SETT $Injection_Voltage
EPS On
SYST:WAIT $DC_Injection_Time
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
STR:ROB:ZOFF 0
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Starting Electrophoresis
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
SYSTem:MESSage "Ramping Cycle."
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SQRR $Run_Voltage $Voltage_Number_Of_Steps $Voltage_Step_Interval
EPS On
SYST:WAIT $Voltage_Ramp_Wait
EPS:SYNC 10
//
// Data Delay
//
SYSTem:MESSage "Waiting for Data."
SYST:WAIT $DC_Data_Delay
//
// CCD beginning data read
//
CAM:SHUT Auto
DAT:SRAT $First_ReadOut_Time $Second_ReadOut_Time
DAT:MODe Binning
SYSTem:MESSage "Starting Data Collection."
DAT:COLL Start
SYST:WAIT $DC_Run_Time
SYSTem:MESSage "Stop Data Collection."
DAT:COLL Stop
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
//LAS Off
//DETH Off
//OVEN Off
//
//
// End Of Module4[0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20;0,0,12,24,36,48,60,72,84,96,108,120,132,144,156,168,180,192,204,216,228;0,11,23,35,47,59,71,83,95,107,119,131,143,155,167,179,191,203,215,227,239][100.0,200.0,300.0,400.0][100.0,200.0,300.0,400.0]3AppliedBiosystems1.05.50.950.99Applied Biosystems[Dye1;540;1, Dye2;568;2, Dye3;595;3, Dye4;615;4][POP6, POP7][36, 50][sequenceStandard]525.0KB_3500_POP7_BDTv3.mobKB.bcpfalse00falsetruefalsetrue20false10000.0800true0.030,15300,100300, 1008,43truetrue4truetruetrue205503true20false20202020falsetrue43020Std_Seq_Assay_xL-POP7 v1.0.0 2021-11-13 12:33:30 -05:00Failed estimation of width curve: ; 2020-10-23 23:59:00.0 2020-02-19 13:10:00.0 M519K5512 2021-11-19 23:59:00.0 2021-11-09 14:49:00.0 2102715 pass Omayra_ZamiaP_plate1 Comment:0m4Xz3qsV5iLP+EX0D68tw==
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